HISTOLOGICAL, HISTOCHEMICAL, MORPHOMETRIC AND MICROBIOLOGICAL INDICATORS OF CECUMIN BROILERS FED WITH DIFFERENT CONTENTS OF PROBIOTICS
G. Kotsyumbas, A. Kostynyuk
This email address is being protected from spambots. You need JavaScript enabled to view it.
Lviv National University of Veterinary Medicine and Biotechnologies named after S. Z. Gzhytskyj,
50 Pekarska str., Lviv 79010, Ukraine
The article displays the results of histological, histochemical and morphometric studies of cecum mucosa in broilers which were fed for 42 days with a row feed with different content of probiotic preparations — “Probion forte” in a dose of 1 g/kg, 0.5 g/kg and “Bio Plus 2B” at a dose of 0.4 g / kg. However, the quantitative microbial composition of cecum contents was investigated at the 42nd day of experiment. At the 15th, 30th and 42nd day of the experiment 10 chickens from each group were taken out of the experiment. After the autopsy the pieces of cecum were selected and fixed in 10 % neutral formalin solution by liquid Carnoy and then filled in paraffin. Histological sections were produced and stained with hematoxylin-eosin, by Stidman; by McManus.
The use of food probiotics in different doses in broiler chickens during their growth and development contributed to the improvement of functional state of the intestines. In the cecum mucosa of chickens of I, II, and III research groups at 42nd day villus height significantly increased to 229.9 mm, 179.08 mm, 215.5 mm and crypt depth — to 45.02 mm, 23.7 mm, 36.9 mm, respectively. The strengthening of the immune barrier of cecum was noted which was reflected in the active population of lymphocytes of the lymph nodes, the relative increase in the number and size of lymphoid structures in the lamina propria of the mucosa. However, the relative increase of volume and number of goblet cells of the villi cecum of poultry research groups and enriching their cytoplasm with neutral glycosaminoglycans and mucopolysaccharides was detected. This contributed to massive mucous biofilm covering the walls of epithelial cells and filling the space between the fibers which was a powerful barrier for penetration of pathogenic and conditionally pathogenic microflora. In the cecum contents in chickens of I, II, III group an increase of microbial genus Lactobacillus (15.3 %, 14. 0 %, 10.7 %) and reducing the number of microorganisms sort E. coli — (22.9% 20 3% 16.5 % respectively) was found compared to control. At the 42nd day of usage of probiotics the microorganisms of genus Salmonella, Campilobacter and Cl. Perfingens were not found.
Keywords: CHICKEN-BROILERS, PROBIOTIC PREPARATIONS, CECUM, VILLI, CRYPTS, GOBLET CELL, MORFOMETRY, HISTOSTRUCTURE, HISTOCHEMISTRY, MIRCROBIAL COMPOSITION
