EFFECT OF L-CARNITINE IN THE COMPOSITION OF THE DIET BULLS ON LIPID CONTENT AND QUALITY OF SPERM
V. А. Koberska
vick_777@ukr.net
Bila Cerkva National Agrarian University, Soborna Square, 8/1,
Bila Cerkva, 09117, Ukraine
Actual problems of modern biological science is the diagnosis of pathological changes in sperm, including oxidative stress. The main targets in the processes of free radical oxidation of sperm is lipids and their fractions. Therefore, changes in the sperm of their contents can be used as biological indicators that determine its quality.
The changes of total lipids in the semen of bulls was investigated during feeding L-carnitine in a secure form under the trade name «Karnipass» (production Loman animal health, Germany) in an amount of 20 g/head and 40 g/head respectively during 75 days. For action supplements the increase of total lipids due to the likely increase in the proportion of phospholipids, lower cholesterol and non-esterified fatty acids bulls sperm. The results showed prolonged effect of additives in 22 days after its application. A negative correlation between the content of phospholipids and cholesterol (r=–0,74), the content of non-esterified fatty acids (r=-–0,52) in the semen of bulls. Changes in the content of total lipids and their relative fractions in the semen of bulls by the action of L-carnitine testify about the activation of anabolic processes, mobilization of lipids as an energy source and the regulation of the lipid phase of the membrane microviscosity in spermatozoa.
Showed a significant increase in activity and survival of sperm by the action of L-carnitine. Improvement of physiological parameters of semen by the action of L-carnitine can be attributed to the optimization of the structure and function of membranes of sperm, reduced lipid peroxidation processes in them, better availability of fatty acids for energy, preservation of the integrity and viability of germ cells. A more cost-effective and efficient feeding dose appeared «Karnipass» in the amount of 20 g/head per day.
Keywords: LIPID COMPOSITION, PHOSPHOLIPIDS, CHOLESTEROL, FATTY ACIDS NEESTERYFIKOVANI, SEMEN, BULLS, L-CARNITINE
