THE EFFECT OF LEAD ON THE ACTIVITY OF ENZYMES OF ANTIOXIDANT PROTECTION AND LIPID PEROXIDATION IN ERYTHROCYTES OF CARP FLAKE
M. Ya. Onyskovets, V.V.Snitinskiy
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Lviv National Agrarian University NAAS; 1 V. Velykyj Str, Dubliany, 80381, Ukraine
It is known that the main mechanism of lead toxicity is the development of oxidative stress as evidenced by disturbances in the pro-and antioxidant blood system as an integral indicator of the body status. In this regard, important is to ascertain the metabolic effects of lead in red blood cells that are among the first to fall under the influence of toxins altered internal environment and possess strong antioxidant defense system. The purpose of our study was to investigate the effect of lead on the activity of antioxidant enzymes and concentration of lipid peroxidation products in erythrocytes of carp flake. In our studies we used a two-year carp flake (Cyprinus carpio L.) with average weight 300–350 g. Each experimental group included in 7 animals. We investigated the influence of lead ions (Pb2+) at a concentration of 0.2, 0.5 and 5 mg/l, corresponding to 2; 5 and 50 maximum permissible concentration (MPC) on the fish organism. Fish was kept in a medium with the addition of lead acetate for 96 h. As a result of the studies we found a significant increase in the activity of antioxidant enzymes during exposure of 2 and 5 MPC lead ions, and inhibition of enzyme activity at 50 MPC, indicating significant stress protective systems against the background of activation of oxidative stress. Installed effects are accompanied by accumulation of lipid peroxidation products (malonic dialdehyde, diene conjugates) in erythrocytes of experimental fish is dose-dependent manner. The data showed that the red blood cells of fish are particularly sensitive to oxidative stress induced by lead ions, so urgent is to develop tools that would have warned accumulation of the free radical reactions products in these cells.
Keywords: LEAD, ERYTHROCYTES, BLOOD, ANTIOXIDANT SYSTEM, LIPID PEROXIDATION PRODUCTS, OXIDATIVE STRESS, CARP FLAKE
