FUNCTIONAL STATUS OF ERYTHRON SYSTEM UNDER THE INFLUENCE OF T-2 TOXIN AND THE POSIBILITY OF CORECTION WITH AEROSIL
О. I. Fedyakova, I.Y. Kotsyumbas
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State Scientific-Research Control Institute of Veterinary Preparations and Food Additives Donetska str., 11, Lviv 79019, Ukraine
Often, changes of structural and functional state of erythron is a diagnostic indicator of the chemical factor influence on the body. Under the normal circumstances the red blood cells are able to partially counteract of various factors, but this resistance weakens with aging and negative factor’s impact, including mycotoxins. Particularly dangerous (due to the wide share in nature) are the toxins of microscopic fungi from Fusarium family, including T-2 toxin, that belongs to the class 1 hazard (LD50 for most species in a single oral administration of 2–10 mg/kg. Since red blood cells are highly sensitive to chemical substances the morpholigicalandfunctional changes allow to set the intensity of toxic influence.
The study was perfomed on Wistar male rats weighing 180–200 g. Two phases of research have been conducted to achieve this goal. The first one was study the effect of T-2 toxin after a single dose administration of 1/10 LD50. The effect of silica on day 4 after toxin administration was studied in the second phase.
The impact of T-2 toxin on certain haematological parameters of the rats’ blood and erythron morphological and functional state was analysed. Depressive impact of T-2 toxin on the system of erythropoesis and protein biosynthesis was established, which leads to decrease in erythrocyte count, corpuscular volume haemoglobin concentration and derived indicators. Reduction in erythrocytes resistance to haemolytic and population redistribution of age fractions caused by accelerated cell aging under the influence of toxicant also found. The positive changes to all the above parameters during the simultaneous introduction of the toxin and silica was studied. Change of haematological parameters and dynamical reticulocytes exit indicates high absorption capacity of silica (aerosil) on T-2 toxin.
Keywords: ERYTHRON, T-2 TOXIN, HAEMATOLOGICAL PARAMETERS, RED BLOOD CELLS, HEMATOCRIT, HAEMOGLOBIN, ERYTHROGRAMS, FRACTIONATION OF ERYTHROCYTES
