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Vorobets PY. The effect of Apis mellifera ligustica royal jelly on certain biochemical blood parameters in rabbits . Bìol Tvarin. 2026; 28 (2): 33–38. DOI: 10.15407/animbiol28.02.033.
https://doi.org/10.15407/animbiol28.02.033
Received 10.06.2026 ▪ Revision 22.07.2026 ▪ Accepted 13.08.2026 ▪ Published online 31.08.2026

The effect of Apis mellifera ligustica royal jelly on certain biochemical blood parameters in rabbits

P. Y. Vorobets*
*This email address is being protected from spambots. You need JavaScript enabled to view it.

Institute of Animal Biology NAAS, 38 V. Stusa str., Lviv, 79034, Ukraine

Royal jelly is a secretion produced by worker honeybees and contains a wide range of biologically active substances, including proteins, lipids, vitamins, minerals, polyphenolic compounds, and other components that account for its physiological effects on the body. The aim of this study was to investigate the effect of royal jelly obtained from Apis mellifera ligustica (an Italian bee breed) on certain biochemical parameters in rabbit blood. During the experiment, parameters of protein, lipid, and mineral metabolism were determined, as well as the activity of specific blood enzymes. A decrease in the activity of aspartate aminotransferase (P<0.05–0.001) and alanine aminotransferase (P<0.01), as well as in cholesterol levels (P<0.05), was observed on the 14^th and 29^th days of the study. On the 29^th day of the experiment, urea levels decreased (P<0.05). At the final stage of the study, an increase in alkaline phosphatase activity and in the levels of total protein, calcium, and phosphorus was observed (P<0.05). The results obtained indicate a positive effect of royal jelly on certain biochemical parameters of rabbits’ blood and confirm the potential for its use as a biologically valuable supplement.

Keywords: royal jelly, rabbits, cholesterol, urea, protein, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase

1. Ahmad S, Campos MG, Fratini F, Altaye SZ, Li J. New insights into the biological and pharmaceutical properties of royal jelly. IntJ Mol Sci. 2020; 21(2): 382. DOI: 3390/ijms21020382.
2. Aminian AR, Khoshniat MT, Khajavirad A, Mahmoudabady M, Rigi MH, Hosseinian S. Nephroprotective impact of royal jelly against rhabdomyolysis-induced kidney damage in rats. IranJ Bas Med Sci. 2025; 28(5): 612–619. DOI: 22038/ijbms.2025.79750.17275.
3. Bagameri L, Botezan S, Bobis O, Bonta V, Dezmirean DS. Molecular insights into royal jelly anti-inflammatory properties and related diseases. Life. 2023; 13(7): 1573. DOI: 3390/life13071573.
4. Balkanska R. Correlations of physicochemical parameters, antioxidant activity and total polyphenol content of fresh royal jelly samples. IntJ Curr Microbiol App Sci. 2018; 7(4): 3744–3750. DOI: 20546/ijcmas.2018.704.421.
5. Bava R, Castagna F, Lupia C, Poerio G, Liguori G, Lombardi R, Naturale MD, Bulotta RM, Biondi V, Passantino A, Britti D, Statti G, Palma E. Hive products: Composition, pharmacological properties, and therapeutic applications. Pharmaceuticals. 2024; 17(5): 646. DOI: 3390/ph17050646.
6. Botezan S, Baci GM, Bagameri L, Pașca C, Dezmirean DS. Current status of the bioactive properties of royal jelly: A comprehensive review with a focus on its anticancer, anti-inflammatory, and antioxidant effects. Molecules. 2023; 28(3): 1510. DOI: 3390/molecules28031510.
7. Caixeta DC, Teixeira RR, Peixoto LG, Machado HL, Baptista NB, de Souza AV, Vilela DD, Franci CR, Salmen Espindola F. Adaptogenic potential of royal jelly in liver of rats exposed to chronic stress. PLoS One. 2018; 13(1): e0191889. DOI: 1371/journal.pone.0191889.
8. Chen YF, Wang K, Zhang YZ, Zheng YF, Hu FL. In vitro anti-inflammatory effects of three fatty acids from royal jelly. Mediat Inflamm. 2016; 2016: 3583684. DOI: 1155/2016/3583684.
9. Collazo N, Carpena M, Nuñez-Estevez B, Otero P, Simal-Gandara J, Prieto MA. Health promoting properties of bee royal jelly: Food of the queens. Nutrients. 2021; 13(2): 543. DOI: 3390/nu13020543.
10. El-Didamony SE, Gouda HIA, Zidan MMM, Amer RI. Bee products: An overview of sources, biological activities and advanced approaches used in apitherapy application. Biotechnol Rep. 2024; 44: e00862. DOI: 1016/j.btre.2024.e00862.
11. El-Hanoun AM, Elkomy AE, Fares WA, Shahien EH. Impact of royal jelly to improve reproductive performance of male rabbits under hot summer conditions. World Rabbit Sci. 2014; 22(3): 241–248. DOI: 4995/wrs.2014.1677.
12. Elnagar SA, Elghalid OA, Abd-Elhady AM. Royal jelly: can it reduce physiological strain of growing rabbits under Egyptian summer conditions? Animal. 2010; 4(9): 1547–1552. DOI: 1017/S1751731110000753.
13. Erzincan R, Caglayan C, Kandemir FM, İzol E, Gür C, İleritürk M. Hepatoprotective effects of royal jelly against vincristine-induced hepatotoxicity in rats: A biochemical and molecular study. Life. 2025; 15(3): 459. DOI: 3390/life15030459.
14. Gasbarri C, Angelini G. Behind the therapeutic effects of royal jelly: Recent advances in the specific properties of 10-hydroxydecanoic acid. Molecules. 2025; 30(13): 2694. DOI: 3390/molecules30132694.
15. Giampieri F, Quiles JL, Cianciosi D, Forbes-Hernández TY, Orantes-Bermejo FJ, Alvarez-Suarez JM, Battino M. Bee products: an emblematic example of underutilized sources of bioactive compounds. JAgric Food Chem. 2022; 70(23): 6833–6848. DOI: 1021/acs.jafc.1c05822.
16. Hamza RZ, Al-Eisa RA, El-Shenawy NS. Possible ameliorative effects of the royal jelly on hepatotoxicity and oxidative stress induced by molybdenum nanoparticles and/or cadmium chloride in male rats. Biology. 2022; 11(3): 450. DOI: 3390/biology11030450.
17. Hidaka S, Okamoto Y, Uchiyama S, Nakatsuma A, Hashimoto K, Ohnishi ST, Yamaguchi M. Royal jelly prevents osteoporosis in rats: Beneficial effects in ovariectomy model and in bone tissue culture model. Evid Based Compl Altern Med. 2006; 3(3): 339–348. DOI: 1093/ecam/nel019.
18. Isidorov VA, Bakier S, Grzech I. Gas chromatographic-mass spectrometric investigation of volatile and extractable compounds of crude royal jelly. JChromatogrB. 2012; 885–886: 109– DOI: 10.1016/j.jchromb.2011.12.025.
19. Kashima Y, Kanematsu S, Asai S, Kusada M, Watanabe S, Kawashima T, Nakamura T, Shimada M, Goto T, Nagaoka S. Identification of a novel hypocholesterolemic protein, major royal jelly protein 1, derived from royal jelly. PLoS One. 2014; 9(8): e105073. DOI: 1371/journal.pone.0105073.
20. Kausar SH, More VR. Royal jelly: organoleptic characteristics and physicochemical properties. Pharm ChemJ. 2019; 6(2): 20–24. Available at: https://www.researchgate.net/publication/332909607_Royal_Jelly_Organoleptic_Characteristics_and_Physicochemical_Properties
21. Kohno K, Okamoto I, Sano O, Arai N, Iwaki K, Ikeda M, Kurimoto M. Royal jelly inhibits the production of proinflammatory cytokines by activated macrophages. Biosci Biotechnol Biochem. 2004; 68(1): 138–145. DOI: 1271/bbb.68.138.
22. Liu JR, Yang YC, Shi LS, Peng CC. Antioxidant properties of royal jelly associated with larval age and time of harvest. JAgric Food Chem. 2008; 56(23): 11447–11452. DOI: 1021/jf802494e.
23. Melliou E, Chinou I. Chemistry and bioactivities of royal jelly. In: Studies in Natural Products Chemistry. Elsevier; 2014; 43: 261–290. DOI: 10.1016/B978-0-444-63430-6.00008-4.
24. Oka H, Emori Y, Kobayashi N, Hayashi Y, Nomoto K. Suppression of allergic reactions by royal jelly in association with the restoration of macrophage function and the improvement of Th1/Th2 cell responses. Int Immunopharmacol. 2001; 1(3): 521–532. DOI: 1016/S1567-5769(00)00007-2.
25. Oršolić N, Jazvinšćak Jembrek M. Royal jelly: biological action and health benefits. IntJ Mol Sci. 2024; 25(11): 6023. DOI: 3390/ijms25116023.
26. Park MJ, Kim BY, Park HG, Deng Y, Yoon HJ, Choi YS, Lee KS, Jin BR. Major royal jelly protein 2 acts as an antimicrobial agent and antioxidant in royal jelly. JAsia Pac Entomol. 2019; 22(3): 684–689. DOI: 1016/j.aspen.2019.05.003.
27. Pasupuleti VR, Sammugam L, Ramesh N, Gan SH. Honey, propolis, and royal jelly: A comprehensive review of their biological actions and health benefits. Oxid Med Cell Longev. 2017; 2017: 1259510. DOI: 1155/2017/1259510.
28. Petrovska I, Salyha Y, Vudmaska I. Statistical Methods in Biological Research. Kyiv, Ahrarna nauka, 2022: 172 p. Available from: https://www.inenbiol.com/images/stories/Rozrobky/Books/2022/Statistika_2022.pdf (in Ukrainian)
29. Ramadan MF, Al-Ghamdi A. Bioactive compounds and health-promoting properties of royal jelly: A review. JFunct Foods. 2012; 4(1): 39–52. DOI: 1016/j.jff.2011.12.007.
30. Rojasawasthien T, Ito T, Okamoto H, Okumura N, Yamashita Takeuchi S, Shirakawa T, Matsubara T, Kokabu S. Sebacic acid promotes osteoblast differentiation. Biosci Biotechnol Biochem. 2025; 89(11): 1572–1578. DOI: 1093/bbb/zbaf128.
31. Sabatini AG, Marcazzan GL, Caboni MF, Bogdanov S, Almeida-Muradian LB. Quality and standardisation of royal jelly. JApiProd ApiMed Sci. 2009; 1(1): 1–6. DOI: 3896/IBRA.4.01.1.04.
32. Salyha Y, Kupynyak N. Heat Stress in Animals. A  Kyiv, Ahrarna nauka, 2024: 320 p. DOI: 10.31073/978-966-540-634-1. (in Ukrainian)
33. Vittek J. Effect of royal jelly on serum lipids in experimental animals and humans with atherosclerosis. Experientia. 1995; 51(9–10): 927–935. DOI: 1007/BF01921742.
34. Vlizlo VV (ed.), Fedoruk RS, Ratych IB. Laboratory Methods of Investigation in Biology, Stockbreeding and Veterinary Medicine. Lviv, Spolom, 2012: 764 p. (in Ukrainian)
35. Vorobets PY, Romanovych MM, Kaplinskyi VV, Tsap MM, Pylypets AZ. Royal jelly: biological properties and production technology. Methodological recommendations. / Institute of Animal Biology NAAS, 2025: 28 p. DOI: 15407/Animal_Biology_978-6-17866-020-8. (in Ukrainian)
36. Xue X, Wu L, Wang K. Chemical Composition of Royal Jelly. In: Alvarez-Suarez JM (ed.). Bee Products-Chemical and Biological Properties. Springer, 2017: 181–190. DOI: 1007/978-3-319-59689-1_8.