Bìol. Tvarin, 2017, Volume 19, Issue 2, pp. 9–15
MALATE DEHYDROGENASE ACTIVITY AND ISOZYME CONTENT IN UTERUS AND OVARIAN TISSUES OF COWS DURING ESTRAL CYCLE
M. M. Akymyshyn
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Institute of animal biology NAAS,
38 V. Stusa str., Lviv 79034, Ukraine
Malate dehydrogenase (MDG) activity and isozyme content in ovarian tissues, follicle antral fluid and endometrium on different stages of folliculogenesis and luteogenesis, and at hypofunction were studied.
It is registered that MDG activity in ovarian tissues is the highest in follicle phase (1.0±0.21 nmol/min×mg of protein), it is lower on 20 % when ovary is in early corpus luteum state and the lowest at state of late corpus luteum (0.6±0.16 nmol/min×mg of protein). In endometrium in active ovarian phase activity of MDG is 0.9–1.3 nmol/min×mg of protein and indexes are on 55.6–69.3 % lower when hypofunction is present. In follicle antral fluid depending on physiological state of ovary activity of MDG is different. In follicles with size less than 4 mm the MDG activity is high (1.6±0.46 nmol/min×mg of protein) at state of follicle growth with size 4–7 mm is lower at states of early and late corpus luteum (0.6–0.7 nmol/min×mg of protein) and it is the lowest (0.4±0.06 nmol/min×mg of protein) when hypofunction is present. In tissues of gonads and antral fluid from ovarian follicles we registered three active MDG proteins, that correspond by elecrtoforetical mobility to two cytosol isozymes (MDG1 and MDG2) and one mitochondrial (MDG3). In ovarian tissue MDG1 and MDG2 content is high (33.7–44.9 %) and MDG3 content is low (18.8–27.1 %), and in endometrium the main part of enzyme falls on MDG2 (36.0–56.7 %) and less — on MDG1 (26.5–35.2 %) and MDG3 (16.7–29.0 %). In antral fluid from follicles content of MDG1 in state of early corpus luteum is the highest (52.5–60.9 %), content of MDG2 is the highest at state of late corpus luteum (43.9–56.1 %), and MDG3 — in follicle growth (27.7–41.8 %). Correlation analysis showed that MDG activity in connection with physiological state change and when hypofunction is present has medium strength (η=0.353), and in endometrium is strong (η=0.799). Content of MDG3 in gonad tissue and MDG2 in endometrium with change of physiological state and when hypofunction is present correlates with medium force (η=0.509 і 0.325, correspondingly). Correlation ratio between size of follicle at early corpus luteum state and activity of MDG2 in antral fluid is curvilinear and has medium strength (η=0.382).
Keywords: MALATE DEHYDROGENASE, ACTIVITY, ISOZYMES, ENDOMETRIUM, OVARIES, FOLLICULOGENESIS, LUTEOGENESIS, COWS
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