Біологія тварин

QUANTITATIVE INSULIN SENSITIVITY CHECK INDEXES IN EARLY POSTPARTUM COWS AND CALVES KEPT IN A BEEF SUCKLER SYSTEM

L. Vranković¹, J. Aladrović¹, I. Pipal², B. Beer Ljubić³, N. Prvanović Babić⁴, Z. Stojević¹

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The aim of our study was to determine indexes of insulin resistance (IR) in early postpartum period in cows and their calves kept in a beef suckler system. The study was conducted on 13 Simmental cows and their calves. We calculated following indexes values: RQUICKI (Revised Quantitative Insulin Sensitivity Check Index) and its modified version RQUICKI_BHB, HOMA (Homeostatic Model Assessment) and QUICKI (Quantitative Insulin Sensitivity Check Index) based on serum concentrations of glucose, insulin, NEFA (non esterified fatty acids) and BHB (β-hydroxybutyrate), sampled 6, 12 and 48h and 7 and 14 d pp. Cows were grouped according to lactation number (2^nd to 4^th lactation, 1^st group; and 5^th to 8^th lactation, 2^nd group) and calves according to gender.

Results showed that RQUICKI was higher in 1^st group of cows (P>0.05) and showed progressive increase during pp period in both groups. In 1^st group of cows RQUICKI was lower 6 and 12 h pp than 14 d p.p. (P=0.01). HOMA was higher in 2^nd group of cows (P>0.05); HOMA was higher 12 h pp than 48 h, 7 and 14 d p.p. (P=0.01) as well as 6 h pp than 7 and 14 d p.p. (P=0.01). QUICKI showed the same trend in both groups, progressive decrease from 6h to 14 d p.p. (P=0.001, 1^st group; P=0.001, 2^nd group). In calves, HOMA was higher in females (P>0.05). In females HOMA was lower 6 h pp than 7 and 14 d p.p. (P=0.03). RQUICKI_BHB was higher in females than in males (P=0.03). RQUICKI_BHB showed progressive decline from 6 h p.p. to 14 d p.p. in females (P=0.004). Changes in trends of IR indexes may help in analysis of decreased sensitivity or responsiveness to the metabolic actions of insulin as well as in determination of metabolic status of animals.

Keywords: COWS, CALVES, EARLY POSTPARTUM PERIOD, INSULIN RESISTANCE, BEEF SUCKLER SYSTEM

1. Bell A.W. Regulation of organic nutrient metabolism during transition from late pregnancy to early lactation. Journal of Animal Science, 1995, vol. 73, issue 9, pp. 2804–2819. https://doi.org/10.2527/1995.7392804x
2. Bossaert P., Leroy J. L. M. R., De Campeneere S., De Vliegher S., Opsomer G. Differences in the glucose-induced insulin response and the peripheral insulin responsiveness between neonatal calves of the Belgian Blue, Holstein-Friesian, and East Flemish breeds. Journal of Diary Science, 2009, vol. 92, issue 9, pp. 4404–4411. https://doi.org/10.3168/jds.2009-2218
3. Cincović M., Kirovski D., Vujanac I., Belić B., Djoković R. Relationship between the indexes of insulin resistance and metabolic status in dairy cows during early lactation. Acta Veterinaria-Beograd, 2017, vol. 67, issue 1, pp. 57–70. https://doi.org/10.1515/acve-2017-0006
4. De Koster J., Hostens M., Hermans K., Van den Broeck W., Opsomer G. Validation of different measures of insulin sensitivity of glucose metabolism in dairy cows using the hyperinsulinemic euglycemic clamp test as the gold standard. Domestic Animal Endocrinology, 2016, vol. 57, pp. 117–126. https://doi.org/10.1016/j.domaniend.2016.06.004
5. Haarstrich D. Evaluation of surrogate indices of insulin sensitivity by means of hyperinsulinemiceuglycemic glucose clamps in dairy cows. In: Insulinsensitivität und Insulinresponse nach einer Langzeit-Supplementation von konjugierten Linolsäuren bei laktierenden Milchkühen. Inaugural disertation. Tierärztliche Hochschule Hannover, 2011.
6. Holtenius P., Holtenius K. A model to estimate insulin sensitivity in dairy cows. Acta Veterinaria Scandinavica, 2007, vol. 49, p. 29. https://doi.org/10.1186/1751-0147-49-29
7. Hostettler-Allen R. L., Tappy L., Blum J. W. Insulin resistance, hyperglycemia, and glucosuria in intensively milk-fed calves. Journal of Animal Science, 1994, vol. 72, issue 1, pp. 160–173. https://doi.org/10.2527/1994.721160x
8. Hugi D., Bruckmaier R. M., Blum J. W. Insulin resistance, hyperglycemia, glucosuria, and galactosuria in intensively milk-fed calves: Dependency on age and effects of high lactose intake. Journal of Animal Science, 1997, vol. 75, issue 2, pp. 469–482. https://doi.org/10.2527/1997.752469x
9. Kahn C. R. Insulin resistance, insulin insensitivity, and insulin unresponsiveness: a necessary distinction. Metabolism — Clinical and Experimental, 1978, vol. 27, issue 12, suppl. 2, pp. 1893–1902. https://doi.org/10.1016/S0026-0495(78)80007-9
10. Kerestes M., Faigl V., Kulcsár A., Balogh O., Földi J., Fébel H., Chilliard Y., Huszenicza G. Periparturient insulin secretion and whole-body insulin responsiveness in dairy cows showing various forms of ketone pattern with or without puerperal metritis. Domesic Animal Endocrinology, 2009, vol. 37, issue 4, pp. 250–261. https://doi.org/10.1016/j.domaniend.2009.07.003
11. Muniyappa R., Lee S., Chen H., Quon M. J. Current approaches for assessing insulin sensitivity and resistance in vivo: advantages, limitations, and appropriate usage. American Journal of Physiology, Endocrinology and Metabolism, 2008, vol. 294, issue 1, pp. E15–E26. https://doi.org/10.1152/ajpendo.00645.2007
12. Opsomer G., Wensing T., Laevens H., Coryn M., Kruif de A. Insulin resistance: the link between metabolic disorders and cystic ovarian disease in high yielding dairy cows? Animal Reproduction Science, 1999, vol. 56, issue 3–4, pp. 211–222. https://doi.org/10.1016/S0378-4320(99)00048-2
13. Perseghin G., Caumo A., Caloni M., Testolin G., Luzi L. Incorporation of the fasting plasma FFA concentration into QUICKI improves its association with insulin sensitivity in nonobese individuals. Journal of Clinical Endocrinology & Metabolism, 2001, vol. 86, issue 10, pp. 4776–4781. https://doi.org/10.1210/jcem.86.10.7902

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