Біологія тварин

EFFECTS OF Α-TOCOPHEROL AND SELENIUM INJECTION ON SERUM CORTISOL IN DAIRY COWS UNDERGOING ABDOMINAL SURGERY

P. Mudroň

Ця електронна адреса захищена від спам-ботів. Вам необхідно увімкнути JavaScript, щоб побачити її.

The present trial was aimed to study the effects of vitamin E and selenium treatment on blood cortisol concentrations in dairy cows stressed by omentopexy.

Twenty dairy cows with left abomasal displacement were used in this study. The cows were randomly divided into two groups. Ten hours before surgery 6 g of DL-α-tocopheryl acetate (6 mg/kg) and 67 mg of sodium selenite (0.1 mg/kg) in volume of 40 ml (Vitaselen^®) were administered subcutaneously to 10 cows; the control animals (n=10) received an equivalent volume of injectable water (40 ml).

The serum vitamin E increased several times ten hours after vitamin E and Se injection and rose continuously to the highest average concentration 21.6 mg/l at hour 24 after the surgery. The highest selenium concentration was seen ten hours after selenium administration with holding the increased concentrations in comparison to initial ones during the whole study. The highest cortisol concentrations were reached at one hour after surgery in the experimental and control group (56.7±28.8 and 65.3±26.1 μg/l, respectively). The ANOVA revealed a significant effect of vitamin E and selenium injection on plasma cortisol (P<0.05).

The decrease of blood cortisol in our study may suggest that vitamin E and selenium supplementation may be an effective method to minimize stress response in dairy cows.

Keywords: SURGICAL STRESS, VITAMIN E, SELENIUM, CORTISOL

1. Abuelo J., Hernández J., Benedito L., Castillo C. The importance of the oxidative status of dairy cattle in the periparturient period: revisiting antioxidant supplementation. Journal of Animal Physiology and Animal Nutrition, 2015, vol. 99, issue 6, pp. 1003–1016. https://doi.org/10.1111/jpn.12273
2. Bergamasco L., Coetzee J. F., Gehring R., Murray L., Song T., Mosher R. A. Effect of intravenous sodium salicylate administration prior to castration on plasma cortisol and electroencephalography parameters in calves. Journal of Veterinary Pharmacology and Therapeutics, 2011, vol. 34, issue 6, pp. 565–576. https://doi.org/10.1111/j.1365-2885.2011.01269.x
3. Breazile J. E. The physiology of stress and its relationship to mechanisms of disease and therapeutics. Veterinary Clinics ofNorth America: Food Animal Practice, 1988, vol. 4, issue 3, pp. 441–480. https://doi.org/10.1016/S0749-0720(15)31025-2
4. Camacho L. E., Meyer A. M., Neville T. L., Hammer C. J., Redmer D. A., Reynolds L. P., Caton J. S., Vonnahme K. A. Neonatal hormone changes and growth in lambs born to dams receiving differing nutritional intakes and selenium supplementation during gestation. Reproduction, 2012, vol. 144, issue 1, pp. 23–35. https://doi.org/10.1530/REP-11-0302
5. Chorfi Y., Girard V., Fournier A., Couture Y. Effect of subcutaneous selenium injection and supplementary selenium source on blood selenium and glutathione peroxidase in feedlot heifers. Canadian Veterinary Journal, 2011, vol. 52, issue 10, pp. 1089–1094.
6. Earley B., Murray M., Prendiville D. J., Pintado B., Borque C., Canali E. The effect of transport by road and sea on physiology, immunity and behaviour of beef cattle. Research in Veterinary Science, 2012, vol. 92, issue 3, pp. 531–541. https://doi.org/10.1016/j.rvsc.2011.04.002
7. Fürll M., Kirbach H., Knobloch B. Glukokortikosteroideinfluss auf die fastenstimulierte Lipolyse und die Leberfunktion bei Kuhen. Tierärztliche Praxis, 1993, vol. 21, pp. 399–403.
8. Gupta S., Kumar H. K., Soni J. Effect of vitamin E and selenium supplementation on concentrations of plasma cortisol and erythrocyte lipid peroxides and the incidence of retained fetal membranes in crossbred dairy cattle. Theriogenology, 2005, vol. 64, issue 6, pp. 1273–1286. https://doi.org/10.1016/j.theriogenology.2005.03.008
9. Herdt T. H., Rumbeiha W., Braselton W. E. The use of blood analyses to evaluate mineral status in livestock. Veterinary Clinics ofNorth America: Food Animal Practice, 2000, vol. 16, issue 3, pp. 423–444. https://doi.org/10.1016/S0749-0720(15)30078-5
10. Hidiroglou N., Laflamme L. F. A dynamic evaluation of the bioavailability of the free and ester forms of vitamin E administered intramuscularly to beef cattle. Veterinary Research, 1993, vol. 24, issue 1, pp. 79–88.
11. Hopster H., Werf Van der J. T. N., Erkens J. H. F., Blokhuis H. J. Effects of repeated jugular puncture on plasma cortisol concentrations in loose-housed dairy cows. Journal of Animal Science, 1999, vol. 77, issue 3, pp. 708–714. https://doi.org/10.2527/1999.773708x
12. Mousaie A., Valizadeh R., Naserian A. A., Heidarpour M., Mehrjerdi H. K. Impacts of Feeding Selenium-methionine and chromium-methionine on performance, serum components, antioxidant status, and physiological responses to transportation stress of Baluchi ewe lambs. Biological Trace Element Research, 2014, vol. 162, issue 1–3, pp. 113–123. https://doi.org/10.1007/s12011-014-0162-x
13. Mudron P., Herzog K., Höltershinken M., Rehage J. Effects of abdominal surgery on thiobarbituric acid reactive substances and plasma anti-oxidative capacity in dairy cows. Journal of Veterinary Medicine Series A, 2007, vol. 54, issue 8, pp. 441–444. https://doi.org/10.1111/j.1439-0442.2007.00992.x
14. Nakao T., Sato T., Moriyoshi M., Kawata K. Plasma cortisol response in dairy cows to vaginoscopy, genital palpation per rectum and artificial insemination. Journal of Veterinary Medicine Series A, 1994, vol. 41, issue 1–10, pp. 16–21. https://doi.org/10.1111/j.1439-0442.1994.tb00060.x
15. Pontes G. C. S., Monteiro P. L. J. Jr., Prata A. B., Guardieiro M. M., Pinto D. A. M., Fernandes G. O., Wiltbank M. C., Santos J. E. P., Sartori R. Effect of injectable vitamin E on incidence of retained fetal membranes and reproductive performance of dairy cows. Journal of Dairy Science, 2013, vol. 98, issue 4, pp. 2437–2449. https://doi.org/10.3168/jds.2014-8886
16. Sordillo L. M., Aitken S. L. Impact of oxidative stress on the health and immune function of dairy cattle. Veterinary Immunology and Immunopathology, 2009, vol. 128, issue 1–3, pp. 104–109. https://doi.org/10.1016/j.vetimm.2008.10.305
17. Sylvester S. P., Stafford K. J., Mellor D. J., Bruce R. A., Ward R. N. Acute cortisol responses of calves to four methods of dehorning by amputation. Australian Veterinary Journal, 1998, vol. 76, issue 2, pp. 123–126. https://doi.org/10.1111/j.1751-0813.1998.tb14544.x

Download full text in PDF