Bìol. Tvarin, 2019, volume 21, issue 2, pp. 11–15


A. Ali1–3, F. Hussein2, E. Mohamed3, H. Maher4

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1Cairo University, Faculty of Veterinary Medicine, Theriogenology and AI Department,
El-Shaheed Gamal El-Deen Afify, Giza Governorate, Cairo, 12211, Egypt
2Alexandria University, Faculty of Veterinary Medicine, Theriogenology and AI Department,
Rasheed El-Mahmoudeya, El Beheira Governorate, Alexandria, 22758, Egypt
3Alexandria University, Medical Research Institute, Medical Biophysics Department,
165 El-Horreya ave., El-Hadara, Alexandria, 21561, Egypt
4Alexandria University, Faculty of Pharmacy, Pharmaceutical Analysis Chemistry Department,
El Sultan Hussein str., Qesm Al Attarin, Alexandria, 21521, Egypt

The objective of the study was to investigate the possibility of applying the Electronic Nose (EN) technology to analyze and detect emanated pheromonal odor changes during estrus cycle in cows. In comparison to gas chromatography (GC) headspace samples analysis and blood hormonal (estradiol and progesterone) analysis using Elisa and RIA. Also to establish a protocol to appoint the proper time of artificial insemination (AI) in cows. The study was conducted on 54 Holstein-Friesian cows. Out of sensors of the EN used in this study, sensor#2 showed the highest response to all measured perineal samples, which adhered perfectly to plasma hormone (E2) levels, and GC analysis of the perineal samples which showed progressive change in acetaldehyde as estrus was approached.


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