The Animal Biology

BT 2019 21 2Bìol. Tvarin. 2018; 20 (1)

https://doi.org/10.15407/animbiol20.01

The Animal Biology, Vol. 20, no. 1, 2018 

FULL ISSUE

 

CONTENTS

Babkina M. M., Vasylchenko O. V., Deriabin O. M., Tarasov A. A., Golovko A. M., 
Palchykovska L. G.
Investigation of the antibacterial action of modified heterocyclic compounds of substituted acridones against Staphylococcus aureus. Page 9–15

Barylo Y. O., Loboiko Y. V.
The comparison of qualitative composition of the muscle tissue of brown trout, rainbow trout and brook trout. Page 16–22G

olopura S. I., Popadiuk B. V., Tsvilihovsky M. I.
The influence of phospholipid-containing preparations on the level of immunoglobulin M in the serum of blood of calves during the period of formation of colostral immunity. Page  23–27

Dziuba V. O., Kuchmenko O. B., Yakoviichuk O. V.
Status of prooxidative-antioxidative balance and activity of Krebs cycle enzymes in liver, heart and kidney tissues for action of various cumulative doses of doxorubicin. Page 28–39

 

Dronyk G. V., Chorna I. V., Rogozynskyi M. S.
Safety assessment of Roundup ready soybean. Page 40–48

Zastulka О.
The ways to reduce contamination of microscopic fungi in bee pollen. Page 49–53

Kozak M. R., Martyn Yu. V., Oliynyk A. V., Kuzmina N. V., Ostapiv D. D., Pajuk O. L., Mitina N. E., Zaichenko O. S., Vlizlo V. V.
Histomorphology of organs and activity of some enzymes in mice after immunization with polymer based on acrylic acid or aluminum hydroxide as adjuvants. Page 54–59

Matiukha І., Vischur O., Broda N., Mudrak D., Sobko G.
Еffectivity of “Antimast” on blood antioxidant profile and lipid peroxides content in dairy cows with subclinical mastitis. Page 60–69

Oziransky Yu., Kolesnyk N., Symon М., Kim S. I., Коnonenko R., Shcherbak S., Fedorenko М.
Indigenous freshwater ichthyofauna of Israel (review). Page 70–96

Pivtorak Y. I., Povroznyk G. V.
Influence of probiotic feed additive “Propoul PLV” on the morpho-productive and quality indexes of quail eggs and derivability of chickens. Page 97–102

Pikhtirova А. V., Ivchenco V. D.
Comparative characteristics of hair cuticle microscopic structure of some species of agricultural animals. Page  103–109

Plys V. M.
Features of microbiocenosis of the poultry organism with the mixed pasteurellosis and ascaridosis disease. Page 110–115

Rublenko I. A., Skripnik V. G.
Study of stability of experimental living vaccine against animal anthrax with strаin UA–07 “Antravak” (18 months after production). Page 116–122

Shtapenko O. V., Gevkan I. I., Slyvchuk Yu. I.
Time-dependent cytotoxicity of cadmium chloride on cells in vitro. Page 123–129 

Bìol. Tvarin, 2018, volume 20, issue 1, pp. 123–129

TIME-DEPENDENT CYTOTOXICITY OF CADMIUM CHLORIDE ON CELLS IN VITRO

O. V. Shtapenko, I. I. Gevkan, Yu. I. Slyvchuk

Institute of Animal Biology NAAS,
38 V. Stus str., Lviv 79034, Ukraine

The dynamics of changes of proliferative and metabolic processes in culture of cow fetus embryonic fibroblast cells at different time of influence of cadmium chloride was evaluated. The dependence of cell growth at time of cultivation with cadmium chloride has been established. The proliferative growth of cells was restored after 3 and 6 h of cultivation cells with cadmium chloride, while the prolonger cultivation to 48 and 72 h caused cytotoxic effect. The decrease of cells viability and proliferation after adding the cadmium chloride to the culture medium was accompanied with the metabolism inhibition in cell culture. At the beginning of the stressful action, the total protein content in conditional medium was similar in control and experimental group, whereas prolonged cultivation cells with cadmium chloride for 24, 48 and 72 h led to significant decrease in total protein compared to the control. The glucose content was significantly higher (P<0.001) in conditional medium of the experimental group that indicating about the insignificant level of glucose consumption through a lower level of biosynthetic processes in treatment cells. The decrease the level of exchange processes by the influence of cadmium chloride is confirmed by the dynamics of changes in the concentration of Сalcium and Рhosphorus. Thus, in experimental group the Рhosphorus content in the conditional medium was significantly higher (P<0.001) at 48 h and 72 h of cultivation compared to the control.
The result of our studies indicate that the decline cells viability and proliferation after adding the cadmium chloride to the culture of cow fetus embryonic fibroblast cells cultivation was observed during 24–72 h of cultivation however, a more pronounced effect was detected in the long-term effect of the compound.

Keywords: CELL CULTURE, CADMIUM CHLORIDE, PROLIFERATIVE ACTIVITY, CYTOTOXICITY

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Bìol. Tvarin, 2018, volume 20, issue 1, pp. 116–122

STUDY OF STABILITY OF EXPERIMENTAL LIVING VACCINE AGAINST ANIMAL ANTHRAX WITH STRАIN UA–07 “ANTRAVAK” (18 MONTHS AFTER PRODUCTION)

I. A. Rublenko1, V. G. Skripnik2
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1Bila Tserkva National Agrarian University,
126 Stavischanska str., Bila Tserkva 09117, Ukraine

2Ukrainian Association of Manufacturers and Distributors
of Veterinary Preparations and Feed Additives,
16 Vasylkivska str, Kyiv 03040, Ukraine

For the prevention of anthrax, various vaccines are developed. The article presents the results of investigations on determination of the experimental live vaccine stability against anthrax from animals of the genus Bacillus anthracis UA–07 “Antravak”.
Experimental vaccines of series 1 and 2 were studied according to the following indices: appearance, presence of foreign impurities, mass fraction of glycerin, pH, amount of live spores, mass spores, contamination, growth pattern, homogeneity, morphology, mobility, capsule formation, harmlessness, residual virulence, immunogenicity activity, storage temperature.
The stability of the studied parameters of the vaccine series 1 and 2 during the study period (6, 12 and 18 months after the making) was established. In the study the mass fraction of glycerin was revealed 29–31%, pH was within 7,5–7,7, the amount of live spores in 1 cm3 was found within 13,48–14,23 million; the vaccine did not cause swelling, skin necrosis, animal death, it was immunogenic for 90 %.
In the study of the parameters of the vaccine series 2, the absence of foreign impurities, bacterial and fungal microflora was revealed.
According to the results of the research, it was found that the experimental vaccine against anthrax from Bacillus anthracis UA–07 “Antravak” strain of series 1 and 2, regardless of storage temperature, was almost constant in the study.

Keywords: ANTHRAX, VACCINE, ANTRAVAC, BACILLUS ANTHRACIS, NECESSITY, SPORI, IMMUNOGENICITY, TEMPERATURE, UNIFORMITY, STABILITY

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Bìol. Tvarin, 2018, volume 20, issue 1, pp. 110–115

FEATURES OF MICROBIOCENOSIS OF THE POULTRY ORGANISM WITH THE MIXED PASTEURELLOSIS AND ASCARIDOSIS DISEASE

V. M. Plys
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State Institute of Crops NAAS of Ukraine,
14 Vernadsky str., Dnipro 49027, Ukraine

The purpose was to study the quantitative composition of microorganisms at the mixed pasteurellosis and ascaridosis disease of poultry. The article presents the results of microbiological monitoring and quantitative composition of microorganisms studied by the mixed pasteurellosis and ascaridosis disease of poultry during 2012–2016. The research was conducted on the basis of the National Scientific Center “Institute of Experimental and Clinical Veterinary Medicine” in the laboratory of epizootology of bacterial diseases of poultry, the State Institution Institute of Agriculture of the Steppe Zone of the National Academy of Agrarian Sciences of Ukraine in the laboratory of veterinary medicine, the private sector and agribusiness of various forms of ownership of the Dnipropetrovsk, Zaporizhzhya, Poltava, Mykolaiv and Vinnytsya regions. The dynamics of microbial contamination of poultry by the mixed pasteurellosis and ascaridosis disease forms depending on the course was studied.
A pathological anatomical dissection of 2,300 corpses of the dead bird was carried out. The material for the study was dead poultry of various species and age groups. Agricultural poultry (chickens, turkeys, geese, ducks) and young (chickens, turkeys, goslings, ducklings), wild migratory (wild ducks), synanthropic (pigeons and doves) and ornamental birds (wavy parrots and young wavy parrots) were subjected to research.
In order to isolate the causative agent of pasteurellosis (cholera), the birds were sown from parenchymatous organs (heart, liver, spleen, gall bladder, kidney) and red bone marrow to simple, enriched and differential diagnostic nutrient media purchased from “Region” (Dnipro), producing countries: Ukraine, India. Bacteriological studies were carried out by methods commonly accepted in microbiology. Labinskaya “Microbiology with the technique of microbiological research”, the determinant of bacteria “Berdzhi”. The biological test was placed on laboratory animals and birds according to generally accepted procedures. Parasitological studies were carried out by helminthoscopy of the test samples by the Füllbörn method.
Out of the pathological material of the fallen bird with a pasteurello-ascaridiosis mixed disease, 734 cultures of microorganisms were isolated. It has been found that the mostly isolated from pathological material composition of the microflora were Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Enterococcus faecalis, Proteus vulgaris, Streptococcus zooepidemicus, Citrobacter amalonaticus, Erysipelothrix rhusiopathiae, Klebsiella pneumoniae. It should be noted that the most common contaminants were these kinds of microorganisms: Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Enterococcus faecalis and Proteus vulgaris, where the first place was occupied by Escherichia coli which averaged about 40 %.
It has been established that a significant number of microorganisms was isolated by acute flow which reached up to 46 % and prevailed by frequency following types of microorganisms: Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Proteus vulgaris, Enterococcus faecalis. In the chronic form of the mixed disease microflora composition was on average 45 % and Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus were dominant.

Keywords: POULTRY, MICROFLORA, MIXED DISEASES, HELMINTHS, CONTAMINATION

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  8. Plys V. M., Fotina T. I. Epizootological monitoring, clinical signs and pathological changes pasteurellosis (Cholera) poultry associations with certain infectious and parasitic diseases. Bulletin of Sumy National Agrarian University, 2014, no. 6 (35), pp. 114–122. (in Ukrainian)
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Bìol. Tvarin, 2018, volume 20, issue 1, pp. 103–109

COMPARATIVE CHARACTERISTICS OF HAIR CUTICLE MICROSCOPIC STRUCTURE OF SOME SPECIES OF AGRICULTURAL ANIMALS

А. V. Pikhtirova, V. D. Ivchenco
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Sumy National Agrarian University,
160 G. Kondratieva str., Sumy 40021, Ukraine, This email address is being protected from spambots. You need JavaScript enabled to view it.

In modern conditions of society development, great attention is paid to the quality of the training future specialists in veterinary medicine as experts who are able to provide qualified assistance in resolving disputed issues that arise at different stages of legal proceedings. Not infrequently there are criminal cases in which animals or their fragments appear, resulting in the need for an expert biologist.
The article presents the results of study of the hair of some farm animal species by scanning electron microscopy. Significant differences in the surface pattern of the hair cuticle of pig, horse, cow, goat and sheep have been revealed. Morphometric investigations of the cuticle scales were carried out using Digimizer digital image analysis software. The thickness of the hair, the frequency of scales arrangement, the average size of scales and their angular characteristics were established.
The largest thickness of the studied samples was in the bristle of pig (188.1 μm), and the smallest — in wool of the Precos breed sheep (15.2 μm). The number of scales per 100 μm of the cuticle surface was as follows: the pig’s bristle — 9 pc., the goat’s hair — 12 pc., the cow’s hair — 13 pc., the horse’s hair — 11/12 pc., the wool of the Hissar breed sheep — 7 pc., the wool of the Precos breed sheep — 9 pc. The average scale size on the surface was 17.5 μm on the pig bristle, 10.6 μm on the goat’s hair, 8.7 μm on the cow’s hair, 10.1–12.1 μm on the horse’s hair, 14.1–14.7 μm on the wool of the Hissar breed sheep, 14.1 μm on the wool of the Precos breed sheep.
The obtained results can be used in the veterinary and sanitary examination to resolve controversial questions on the identification of hair and the attribution of samples to specific farm animal species.

Keywords: HAIR, WOOL, ANIMALS, SCANNING ELECTRON MICROSCOPY, MORPHOMETRIC INDICATORS

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